method

[Nisus]

• I swab the laboratory bench with ethanol (70%)
• I cut a small piece of cauliflower – roughly a centimetre across-with a sharp scalpel.
• I place it in a Petri dish using sharp cuts, divide the piece of cauliflower into three.
• I carefully place the pieces of cauliflower, or explants into 50cm3 of sodium hypochlorite solution in a beaker to sterilise them.
• I left it for 10 minutes.
• I undid the ***** cap, then flame the neck of the bottle of the sterile distilled water provided.
• I poured out 50 cm3 of sterile distilled water into three 100 cm3 beakers.
• I flame the neck of the bottle and replace the cap.
• I dipped the end of a pair of forceps into a beaker of ethanol, keeping this well away from the flame.
• I passed the forceps through a blue Bunsen flame to ignite the ethanol and sterilise them.
• I allowed the forceps o cool and then aseptically transfer the explants to a beaker of sterile water. I left it for one minute.
• I rinse the explants twice more in the remaining two beaker of sterile distilled water. This rinsing process is critical to the growth of the explants. I left it in the beaker until required.
• to the hand opposite I wrote with I removed the aluminium foil and cotton wool plug from the test tube of growth medium.
• I flamed the neck of the tube.
• I was using a pair of sterile forceps then I carefully but quickly transfer one of the explants onto the surface of the growth medium in the tube.
• I carefully flame the neck of the tube and replaced the cotton wool.
• I covered the cotton wool with aluminium foil.
• I repeated stage 15 to 17 with the other two explants. I transferred them aseptically to two further tubes of growth media.
• I kept the explants in a warm light place.
• The growth of the explants should be visible after about 10 days.
• In the tube, aseptic technique has not been observed, and the explant has not grown.
• My healthy explants and contaminated explants compared.

[mochatolatte]

And why must we know this?

[Rachz]

And why must we know this?

My Thoughts precisely